Type 2 diabetes is no longer a problem isolated to high-income countries. The prevalence of type 2 diabetes has almost doubled over the past decades, from 4.7% to 8.5%, with the fastest rise in low-and-middle income countries (LMICs).1,2

Neuropathy is a serious complication of type 2 diabetes, particularly in LMICs, and distal peripheral neuropathy (DPN) is the most common type. Standard methods of diagnosing DPN include nerve conduction studies and skin biopsies; however, clinical symptom scales and monofilament testing are more commonly used in primary care settings.3,4 The use of vibration perception threshold (VPT) testing has also been validated as a method of diagnosing DPN.5 Studies from LMICs have showed hospital and community prevalence rates ranging between 20% and 60%.6,7 A cross-sectional study conducted in Peru demonstrated a hospital prevalence of 57%.8

DPN is often poorly managed in LMICs due to lack of resources. Approximately 25% of patients with DPN will develop foot ulcers, many of which then proceed to amputations, which forebode a high mortality rate.9,10 In addition to the medical burden, DPN among cases of type 2 diabetes poses a significant financial burden on patients and their families. A cost-analysis study in Peru reported costs of US$ 74.5 million for high-risk individuals with diabetes in a single year.11

Given such a high hospital prevalence rate of DPN among cases of type 2 diabetes in Peru, there is a need to determine the rate of this complication at the population level. Therefore, this study aimed to determine the prevalence of DPN in a community-based setting in Peru. Association of DPN with abnormal glycemic status was also assessed.

METHODS

Setting and study population

This was a population-based, cross-sectional study conducted in Tumbes, a semi-urban area in the north of Peru. Details about participant selection and procedures have previously been reported.12 Briefly, based on the most recent census data in the area, participants were selected using a sex-stratified random sampling method.

Inclusion criteria were age between 30 and 69 years, full time residency in the study area (≥6 months), and ability to provide informed consent. Those bedridden, or with physical disabilities preventing anthropometric measurements, as well as pregnant women, were excluded. One participant from each household was selected to avoid clustering.

Outcome variable

The outcome of interest was DPN assessed by VPT testing. VPT was measured with a biothesiometer (Diabetik Foot Care India Pvt Ltd, Chennai, India). Participants were asked to lie in the supine position, and the stylus of the biothesiometer was applied perpendicular to the pulp on the plantar surface of the hallux of both feet. The amplitude of the vibration was gradually increased until the participant could detect the vibration. This process was performed in triplicate for each foot. The participant was considered to have DPN if the average of the three measurements in either foot was ≥25 mV.13 In addition, abnormal VPT was also included and defined as VPT≥10mV but <25mV. 14

Exposure variable

The exposure of interest was glycemic status (normal, dysglycemia, and type 2 diabetes). Type 2 diabetes was defined by the oral glucose tolerance test criteria as per the World Health Organization threshold (fasting glucose ≥126mg/dL (≥7.0 mmol/L) or 2-hour plasma glucose ≥200mg/dL (≥11.1 mmol/L)). Self-reported type 2 diabetes diagnosis by a physician and current treatment for type 2 diabetes were also used in the definition. Dysglycemia was defined as either the presence of impaired glucose tolerance or impaired fasting glucose, both of which were defined according to criteria recommended by the World Health Organization.

Individuals who were previously unaware of their glucose status and had a fasting glucose ≥126mg/dL (≥7.0 mmol/L) or 2-hour plasma glucose ≥200mg/dL (≥11.1 mmol/L) were considered as having undiagnosed type 2 diabetes. On the other hand, those with type 2 diabetes and aware of their condition were categorized as controlled cases if the result of glycated hemoglobin (HbA1c) was <48 mmol/mol (<6.5%).

Other variables

Some variables were also assessed for population description as well as potential confounders in the association of interest. Among socio-demographic variables were age, sex, education level, socioeconomic status, and health insurance. Among behavioral variables were daily smoking, self-reported and based on the consumption of at least one cigarette per day, and alcohol consumption, assessed by the Alcohol Use Disorder Identification Test (AUDIT), with a score of ≥4 considered positive for men, ≥3 for women.15 Anthropometric measures collected were body mass index, waist circumference, and blood pressure (See Table 1 for further details).

Table 1. Characteristics of the study population by glucose status (n=1,607)
Characteristic Glucose Status
Normal Dysglycemia Diabetes P-value
Sex (n=1,159) (n=272) n=176)
Men 612 (52.8%) 112 (41.2%) 74 (42.1%) <0.001
Age:
<40 372 (32.1%) 54 (19.9%) 14 (7.9%) <0.001
40-49 354 (30.5%) 81 (29.8%) 45 (25.6%)
50-59 264 (22.8%) 76 (27.9%) 69 (39.2%)
≥60 169 (14.6%) 61 (22.4%) 48 (27.3%)
Education level:
Primary 336 (29.0%) 102 (37.5%) 80 (45.4%) <0.001
Secondary 559 (48.2%) 116 (42.7%) 73 (41.5%)
Superior 264 (22.8%) 54 (19.8%) 23 (13.1%)
SES based on assets:
Low 370 (31.9%) 100 (36.8%) 68 (38.6%) 0.29
Middle 408 (35.2%) 89 (32.7%) 53 (30.1%)
High 381 (32.9%) 83 (30.5%) 55 (31.3%)
Health insurance:
No 109 (9.4%) 20 (7.4%) 11 (6.3%) 0.26
Daily smoking:
Yes 72 (6.2%) 12 (4.4%) 8 (4.6%) 0.40
Alcohol use disorder:
AUDIT (+) 106 (9.2%) 7 (2.6%) 8 (4.6%) <0.001
BMI:
<25 kg/m2 341 (29.4%) 47 (17.3%) 37 (21.0%) <0.001
25-29.99 kg/m2 514 (44.4%) 111 (40.8%) 81 (46.0%)
≥30 kg/m2 304 (26.2%) 114 (41.9%) 58 (33.0%)
Waist circumference:
<90 cm 450 (38.8%) 76 (27.9%) 44 (25.0%) <0.001
90–99 cm 460 (39.7%) 98 (36.0%) 68 (38.6%)
100+ cm 249 (21.5%) 98 (36.1%) 64 (36.4%)
Hypertension:
Yes 252 (21.7%) 93 (34.2%) 72 (40.9%) <0.001

SES – Socioeconomic status, AUDIT – Alcohol use disorder identification test, BMI – Body mass index

Procedures

Fieldworkers went from house to house to recruit participants. Written informed consent was obtained before undergoing study procedures. Socio-demographic information, medical and familial history, and behavioral variables were collected and recorded by trained staff on tablets in an application built using Open Data Kit (ODK, University of Washington Department of Computer Science and Engineering, Seattle, WA, USA).

Then, anthropometric measurements (ie, weight, height, waist circumference) and blood pressure were obtained. VPT testing was then conducted using a biothesiometer as detailed earlier. Trained laboratory staff collected a fasting glucose blood sample after consent was obtained and another blood sample two hours after a 75g glucose load was administered. A Cobas Modular Platform and Roche Diagnostic reagents were used to analyze glucose levels. HbA1c was assessed using high-performance liquid chromatography (D10-BIORAD, Germany), which is traceable to the National Glycohemoglobin Standarization Program.

Statistical analysis

Analysis was performed using STATA 15.0 for Macintosh (Stata Corp, College Station, TX, US). Study population characteristics were tabulated by means and standard deviations (SD) for continuous variables and percentages for categorical variables. Chi-squared tests were used to compare categorical variables, and p-values <0.05 were considered significant.

To determine the strength of the association between variables of interest, Poisson regression models with robust variance were used.16 Crude and adjusted models were generated, the latter controlling for sex, age, education level, socioeconomic status, daily smoking, alcohol use disorder, waist circumference, and high blood pressure levels.

Ethics

The protocol, consent, and questionnaires received approval from the Institutional Review Board at the Universidad Peruana Cayetano Heredia in Lima, Peru and the London School of Hygiene and Tropical Medicine, London, UK.

RESULTS

Population characteristics

A total of 1,609 participants were enrolled in the study. However, only 1,607 were analyzed, as 2 did not have OGTT results. The mean age of participants was 48.2 (standard deviation (SD)=10.6); 809 (50.3%) were women, and 341 (21.2%) had 12+ years of education.

Out of the 1607 individuals, 176 (11.0%; 95% confidence interval (CI)=9.5–12.6) had type 2 diabetes and 272 (16.9%; 95% CI=15.1–18.9) presented dysglycemia. A greater proportion of individuals with type 2 diabetes and dysglycemia were women compared to those with normal glucose status (P<0.001). Those with type 2 diabetes were older (P <0.001), had a lower level of education (P<0.001), higher waist circumference (P<0.001), and higher prevalence of high BP (P<0.001) compared to those with dysglycemia and normal glucose status (See details in Table 1).

Finally, among those with type 2 diabetes, 105 (59.7%) were aware of their diagnosis before the study. Among these participants, 94 (89.5%) reported being on treatment, while 30 (28.6%) had their type 2 diabetes appropriately controlled.

Prevalence of DPN and glucose status

A total of 309/1607 participants (19.2%, 95% CI=17.3–21.2) had results compatible with DPN, and 933 (58.1%; 95% CI=55.6–60.5) had abnormal biothesiometer results. Mean age was higher in the DPN group (57.2 years, SD=8.0) compared to the abnormal biothesiometer result and normal group (47.9 years, SD=10.0 and 41.2 years, SD: 8.1, respectively; P<0.001). A greater proportion of individuals with DPN were men (59.8%) compared to those with abnormal and normal results (P<0.001). Details of sociodemographic variables assessed according to biothesiometer result are shown in Table 2.

Table 2. Characteristics of the study population by biothesiometer results
Characteristics Biothesiometer result
Normal (<10mV) Abnormal (10-25 mV) Neuropathy (>25 mV) P-value
Sex (n=365) (n=935) (n=309)
Men 148 (18.5%) 476 (59.6%) 175 (21.9%) <0.001
Women 217 (26.8%) 459 (56.7%) 134 (16.5%)
Age:
<40 191 (43.3%) 242 (54.9%) 8 (1.8%) <0.001
40-49 115 (24.0%) 314 (65.4%) 51 (10.6%)
50-59 51 (12.5%) 236 (57.7%) 122 (29.8%)
>60 8 (2.9%) 143 (51.2%) 128 (45.9%)
Education level:
<7 years 53 (10.2%) 294 (56.7%) 172 (33.1%) <0.001
7–11 years 188 (25.1%) 443 (59.2%) 118 (15.7%)
12+ years 124 (36.4%) 198 (58.1%) 19 (5.6%)
Socioeconomic status:
Low 94 (17.4%) 323 (59.8%) 123 (22.8%) < 0.001
Middle 124 (22.6%) 319 (58.0%) 107 (19.4%)
High 147 (28.3%) 293 (56.5%) 79 (15.2%)
Health insurance:
No 37 (26.4%) 76 (54.3%) 27 (19.3%) 0.51
Yes 328 (22.3%) 859 (58.5%) 282 (19.2%)
Daily smoking:
No 351 (23.1%) 884 (58.3%) 282 (18.6%) 0.02
Yes 14 (15.2%) 51 (55.4%) 27 (29.4%)
Alcohol use disorder:
AUDIT (-) 337 (22.7%) 863 (58.0%) 288 (19.4%) 0.87
AUDIT (+) 28 (23.1%) 72 (59.5%) 21 (17.4%)
BMI:
<25 kg/m2 88 (20.7%) 245 (57.7%) 92 (21.6%) 0.002
25-29.99 kg/m2 155 (21.9%) 443 (62.6%) 110 (15.5%)
>30 kg/m2 122 (25.6%) 247 (51.9%) 107 (22.5%)
Waist circumference:
<90 cm 155 (27.2%) 326 (57.2%) 89 (15.6%) <0.001
90-99 cm 140 (22.3%) 386 (61.5%) 102 (16.2%)
100+ cm 70 (17.0%) 223 (54.3%) 118 (28.7%)
Hypertension:
No 309 (25.9%) 694 (58.2%) 189 (15.9%) <0.001
Yes 56 (13.4%) 241 (57.8%) 120 (28.8%)
Glucose status:
Normal 283 (24.4%) 699 (60.3%) 177 (15.3%) < 0.001
Dysglycemia 63 (23.2%) 155 (57.0%) 54 (19.8%)
Diabetes 19 (10.8%) 79 (44.9%) 78 (44.3%)

Prevalence of DPN was 44.3% among individuals with type 2 diabetes, 19.8% among those with dysglycemia, and 15.3% among those with normal glucose status (Table 2). In our multivariable model, after controlling for sex, age, education level, socioeconomic status, daily smoking, alcohol use disorder, waist circumference, and hypertension status, type 2 diabetes, but not dysglycemia, was associated with an increased prevalence of DPN compared to the normal glucose status group (PR=1.28, 95% CI 1.13-1.45, Table 3).

Table 3. Association between glucose status and diabetic peripheral neuropathy – crude and adjusted regression models
Biothesiometer result Crude Model Model 1* Model 2†
Abnormal vs. normal (n=1,298) PR (95% CI) PR (95% CI) PR (95% CI)
Normal 1 (Reference) 1 (Reference) 1 (Reference)
Dysglycemia 1.00 (0.91-1.10) 0.95 (0.86-1.03) 0.94 (0.86-1.03)
Diabetes 1.13 (1.02-1.26) 1.05 (0.95-1.16) 1.03 (0.93-1.15)
Neuropathy vs. normal (n=674) PR (95% CI) PR (95% CI) PR (95% CI)
Normal 1 (Reference) 1(Reference) 1(Reference)
Dysglycemia 1.20 (0.96-1.51) 1.04 (0.88-1.24) 1.01 (0.84-1.20)
Diabetes 2.09 (1.80-2.43) 1.31 (1.17-1.48) 1.28 (1.13-1.45)

PR – prevalence ratio, CI – confidence interval
*Adjusted for sex, age, education level, and socioeconomic status
†Adjusted for sex, age, education level, socioeconomic status, smoking, alcohol, waist circumference, and hypertension

Awareness of type 2 diabetes status, duration of type 2 diabetes, and DPN

Among patients with type 2 diabetes, a higher proportion of participants with a previous diagnosis had DPN compared to those who were unaware of their diagnosis (57.1% vs. 24.7%, P<0.001). However, there was no difference in the prevalence of DPN when comparing individuals with type 2 diabetes on treatment to those not on treatment (59.1% vs. 41.7%, P=0.40).

Duration of disease was available for the 105 participants aware of their diabetes diagnosis. The mean duration was 6.3 (SD=6.1, range=0–32) years. DPN was present in 25/55 (45.5%) individuals with <5 years of disease, whereas it was present in 30/50 (70.0%) individuals with ≥5 years of disease (P=0.03).

DISCUSSION

Main findings

A significant association between type 2 diabetes and DPN, even after accounting for potential confounders, is reported in this manuscript. We also found a lower prevalence of DPN in the community compared to the hospital prevalence found in previous studies in Peru (35.8% vs. 56.6%), which is expected given that participants from hospitals tend to be more ill and present with more severe forms of disease. Additionally, there were a large number of participants with signs compatible with non-diabetic neuropathy, and a large number of undetected cases of diabetes in the community.

Comparison with previous studies

Our DPN prevalence estimate, based on a community-based survey, matches the global prevalence of DPN reported in a meta-analysis conducted in 2016.17 This meta-analysis pooled various hospital and community-based studies together, including two separate studies conducted in Brazil and Mexico. DPN prevalence was found to be 26.5% in an outpatient setting in Brazil and 40.8% in a community setting in Mexico.18,19 Besides the studies mentioned above, there is very little additional data regarding prevalence of DPN in Latin American countries. It is important to recognize that, though a global prevalence value of DPN exists, rates vary throughout the globe, with 77% of people with DPN living in low-middle income countries. This distribution could be attributed to genetic predisposition, lifestyle behaviors, level of glycemic control, duration of asymptomatic hyperglycemia, and poor access to effective treatment.17,20,21 Therefore, more studies like this are necessary in Latin America in order to understand the epidemiology and impact of DPN in this region.22

The variability in DPN prevalence as reported in the aforementioned systematic review could also be due to differences in diagnostic methods and cutoff criteria used. Tools used to diagnose DPN range from symptom scales such as the Neuropathy Disability Score, Neuropathy Symptom Score, Michigan Neuropathy Screening Instrument,23 to physical exam procedures like the Semmes-Weinstein Monofilament Examination and VPT testing, to more advanced techniques that are typically gold-standard for research studies: electromyography and nerve conductions studies.24 The accuracy of these tests varies, which can impact prevalence rates. In this study, we used VPT to determine neuropathic status. Although a valid method, the sensitivity and specificity are lower when compared to nerve conduction studies; therefore, we could possibly be underestimating the number of DPN cases.5,25 While it is important to consider the practicality of applying a diagnostic method to a target population, an alternative methodology that would improve our study would be to determine neuropathy with a gold-standard technique.

The high percentage of abnormal biothesiometer results (VPT≥10mV but <25 mV) found in participants from all three groups (normal, dysglycemia, type 2 diabetes) is important to note. We also found that a portion of normal controls and individuals with dysglycemia had results compatible with DPN. Both findings highlight the importance of considering other causes of nerve damage (ie, B12 deficiency, hypothyroidism) and confounding factors (ie, age, obesity).

Alternatively, the fact that some individuals with dysglycemia had results compatible with DPN could point to neuropathy as an early sign or risk factor for disease. A growing body of literature has linked prediabetes (ie, dysglycemia) and obesity with increased risk for DPN and non-diabetic neuropathy.26 However, damage is thought to involve mainly small nerve fibers, which would not explain the large nerve fiber damage detected by VPT that we found. Therefore, although dysglycemia is a risk for developing neuropathy because it progresses to type 2 diabetes, it cannot be the sole cause.27 This latter theory fits the results we found in our regression model, thus corroborating the fact that multiple factors (ie, abnormal glucose levels, obesity, age, and hypertension) contribute to the vessel damage that subsequently manifests as nerve damage seen in individuals with dysglycemia.

Other relevant results

The results of this study emphasize the need for greater surveillance in screening for type 2 diabetes and its complications in the community. Additionally, it highlights the need for better implementation of current screening practices. Many participants were unaware they had type 2 diabetes prior to the study, a phenomenon that has been found in other population-based studies.28 However, this value was much higher in Peru compared to other countries; 17% of individuals with type 2 diabetes had findings compatible with neuropathy at the time of diagnosis.20,21 The high percentage of people with undiagnosed type 2 diabetes and DPN in Peru ultimately indicates a need for earlier detection. However, it also points to neuropathy as a potential bridge between diagnosed and undiagnosed cases in the community. The significantly higher number of people aware of diabetes who also had neuropathy compared to those unaware of type 2 diabetes suggests that neuropathy may be a prompter for people in the community to get screened for type 2 diabetes. Perhaps in resource-constrained settings, detection of neuropathy can be a gateway to type 2 diabetes diagnosis and getting proper treatment. Future studies are needed to investigate causes for delay in type 2 diabetes detection, and perhaps one way to approach this would be to characterize what symptoms people in resource-constrained settings initially present with. Learning how people initially present with type 2 diabetes could provide future direction on how to improve this delay in detection and care.

The large number of uncontrolled cases of type 2 diabetes demonstrates the difficulty of achieving glycemic control among individuals in the community. In large randomized controlled trials, strict glycemic control has been shown to prevent and improve neuropathy in type 1 diabetes mellitus, but has been less evident for type 2 diabetes.29 A hypothesized reason is that the presence of other risk factors (ie, hypertension, obesity, hyperlipidemia, chronic inflammation) in individuals with diabetes adds additional mechanisms that contribute to vascular damage leading to neuropathy symptoms. Nevertheless, glycemic control is still an important aspect of type 2 diabetes control, as poor glucose control contributes to increased complication rates, and complications from neuropathy (ie, foot ulcers and deformities) are exacerbated by poor glucose control.30 Therefore, glycemic control can be a major area healthcare workers can focus on in clinical practice to improve outcomes of type 2 diabetes in LMICs such as Peru.

Characterizing DPN in a community setting is important, as data will help guide future interventions and policy to improve health outcomes. Additionally, the results highlight the need for better implementation of clinical screening practices for DPN (ie, at-home foot exams, regular check-ups with annual foot exams using appropriate tools, comprehensive diabetic foot care programs) in resource-constrained settings. Finally, these results contribute to the growing body of knowledge about DPN in South America, an area where data is relatively scarce.

Strengths and limitations

This study was a community-based survey and thus included a large sample size and avoided biases associated with hospital-based studies. Limitations, however, are present. Possible selection bias can be present, as we only selected participants between ages 30-69 from a certain region in Peru.

VPT has been validated as a tool for diagnosing neuropathy; however, screening guidelines recommend using multiple modalities to characterize DPN. By using VPT alone, we are primarily assessing for large nerve fiber damage and may miss detecting early manifestations of DPN, which involve small nerve fibers. Thus, some cases of neuropathy could be missed.27 As mentioned earlier, VPT is less sensitive than the gold standard, which could underestimate prevalence further. Additionally, we did not inquire into other causes of neuropathy besides type 2 diabetes. Although OGTT is one of the gold standards for diagnosing diabetes, WHO and ADA guidelines recommend two assessments with any blood test to diagnose type 2 diabetes. By only using one OGTT assessment to classify individuals in this study, some misclassification may arise.

CONCLUSIONS

We detected a high prevalence of DPN in the community, although lower than hospital-based studies. The high prevalence of DPN along with the high amount of undiagnosed and uncontrolled cases of type 2 diabetes demonstrate a need for earlier detection, stricter glycemic control, and studies investigating barriers to care in order to improve outcomes of disease, especially in LMICs like Peru.


Acknowledgements

None.

Funding

This work was supported by a grant received through the Research Training Fellowship in Public Health and Tropical Medicine of Antonio Bernabe-Ortiz (103994/Z/14/Z), funded by the Wellcome Trust.

Competing interests

The authors completed the Unified Competing Interest form at http://www.icmje.org/coi_disclosure.pdf (available upon request from the corresponding author), and declare no conflicts of interest.

Correspondence to:

Meera F. Iyengar, BA, MDc
12514 Lynx Lane
Sugar Land, TX, 77478
USA
[email protected]